Molecular analysis of Fungi: Malasseziarestricta from Felidae

A total of 9 samples wild cat Felischausfurax (de Winton, 1898) and 13 (11 positives) domestic Feliscattus (Linnaeus, 1758) belong to Family Felidae. All cats were trapped used hair skin scrapings by forceps surgical blades. The areas the collection were: Mosul province (north Iraq); Baghdad, Al-Rashidiya, Tharthar, Nahrawan, AL-Mahmoudiya (middle Iraq) AL-Haretha (south Iraq). current study revealed that sensitive specific PCR assay allowing rapid reliable identification Malasseziarestricta fragment size amplified was 500bp in ITS1 gene one sample cats. recorded a new strain Malasseziarestrictathat called AF2013 "small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1", complete 5.8S sequence. Which inserted GenBank: MW376484.1 from for first time Iraq. Sequencing close matching phylogenetic tree an isolate Korea (CP030254). compression performed using NCBI – based nucleotides website. Keywords: Dermatitis, Cutaneous microflora, fungi, Genotype, Malasseziarestricta.